Detailed information of TA system TA087569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 253205..253830
• Replicon: chromosome
• Accession: NZ_CP024289
• Organism: Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A5960_RS01305
• Protein ID: WP_000911330.1
• Coordinates: 253432..253830 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A5960_RS01300
• Protein ID: WP_000450524.1
• Coordinates: 253205..253432 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5960_RS01275	249008..249478	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
A5960_RS01280	249478..250050	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A5960_RS01285	250196..251074	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A5960_RS01290	251091..252125	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A5960_RS01295	252338..253051	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A5960_RS01300	253205..253432	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A5960_RS01305	253432..253830	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A5960_RS01310	253977..254840	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
A5960_RS01315	254855..256870	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
A5960_RS01320	256944..257642	+	699	WP_000679823.1	esterase	-
A5960_RS01325	257752..257952	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T87569 WP_000911330.1 NZ_CP024289:253432-253830 [Escherichia coli O178:H19]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T87569 NZ_CP024289:253432-253830 [Escherichia coli O178:H19]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT87569 WP_000450524.1 NZ_CP024289:253205-253432 [Escherichia coli O178:H19]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT87569 NZ_CP024289:253205-253432 [Escherichia coli O178:H19]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33