Detailed information of TA system TA087568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 79443..79968
• Replicon: plasmid unnamed5
• Accession: NZ_CP024287
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CCF14_RS27585
• Protein ID: WP_097492536.1
• Coordinates: 79663..79968 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: CCF14_RS27580
• Protein ID: WP_000813630.1
• Coordinates: 79443..79661 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS27555	74601..75500	+	900	WP_000963206.1	nucleotide-binding protein	-
CCF14_RS27560	75550..77775	-	2226	WP_000698737.1	phage T7 exclusion protein	-
CCF14_RS27565	77777..78865	-	1089	WP_000952217.1	transcriptional repressor PifC	-
CCF14_RS27580	79443..79661	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CCF14_RS27585	79663..79968	+	306	WP_097492536.1	type II toxin-antitoxin system toxin CcdB	Toxin
CCF14_RS27590	79969..80775	+	807	WP_024197775.1	site-specific integrase	-
CCF14_RS27600	81462..82193	-	732	WP_000504263.1	replication initiation protein	-
CCF14_RS27605	82814..84019	+	1206	WP_001401628.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib	-	1..124142	124142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11722.47 Da
• Isoelectric Point: 5.7116

>T87568 WP_097492536.1 NZ_CP024287:79663-79968 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPEVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T87568 NZ_CP024287:79663-79968 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGATATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGAGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8354.27 Da
• Isoelectric Point: 4.5203

>AT87568 WP_000813630.1 NZ_CP024287:79443-79661 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT87568 NZ_CP024287:79443-79661 [Escherichia albertii]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CEF5