Detailed information of TA system TA087566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 39928..40475
• Replicon: plasmid unnamed5
• Accession: NZ_CP024287
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CCF14_RS27360
• Protein ID: WP_000969983.1
• Coordinates: 40194..40475 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5JBQ7
• Locus tag: CCF14_RS27355
• Protein ID: WP_000079943.1
• Coordinates: 39928..40197 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS27310	35582..35794	+	213	WP_001311693.1	hypothetical protein	-
CCF14_RS27315	36037..36498	+	462	WP_001233871.1	thermonuclease family protein	-
CCF14_RS27320	36544..36753	+	210	WP_001333231.1	hemolysin expression modulator Hha	-
CCF14_RS27325	36791..37364	+	574	Protein_41	DUF2726 domain-containing protein	-
CCF14_RS27330	37603..37863	+	261	WP_000083818.1	replication regulatory protein RepA	-
CCF14_RS27340	38089..38163	+	75	WP_001365705.1	RepA leader peptide Tap	-
CCF14_RS27345	38156..39013	+	858	WP_157760426.1	incFII family plasmid replication initiator RepA	-
CCF14_RS27355	39928..40197	+	270	WP_000079943.1	hypothetical protein	Antitoxin
CCF14_RS27360	40194..40475	+	282	WP_000969983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCF14_RS27365	40521..41369	+	849	WP_001058012.1	3'-5' exonuclease	-
CCF14_RS27370	41486..41968	+	483	WP_001311056.1	hypothetical protein	-
CCF14_RS27375	42090..42326	+	237	Protein_49	transposase	-
CCF14_RS27385	42649..43346	-	698	Protein_50	IS1 family transposase	-
CCF14_RS27395	43448..43771	-	324	WP_001175593.1	helix-turn-helix domain-containing protein	-
CCF14_RS27400	43877..45010	+	1134	WP_001089730.1	permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib	-	1..124142	124142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10874.52 Da
• Isoelectric Point: 7.3987

>T87566 WP_000969983.1 NZ_CP024287:40194-40475 [Escherichia albertii]
MMEIFWTMLASQDRKHIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T87566 NZ_CP024287:40194-40475 [Escherichia albertii]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCATATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10272.57 Da
• Isoelectric Point: 7.2057

>AT87566 WP_000079943.1 NZ_CP024287:39928-40197 [Escherichia albertii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLSHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT87566 NZ_CP024287:39928-40197 [Escherichia albertii]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGCCAGCAACTTGAGCGCGGTGATGTGCTTTCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A622US83