Detailed information of TA system TA087559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 36523..37057
• Replicon: plasmid unnamed4
• Accession: NZ_CP024286
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CCF14_RS26610
• Protein ID: WP_099588230.1
• Coordinates: 36523..36813 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CCF14_RS26615
• Protein ID: WP_099588231.1
• Coordinates: 36803..37057 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS26600	35618..36118	-	501	WP_157760419.1	GNAT family N-acetyltransferase	-
CCF14_RS26605	36200..36360	-	161	Protein_26	IS256 family transposase	-
CCF14_RS26610	36523..36813	-	291	WP_099588230.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCF14_RS26615	36803..37057	-	255	WP_099588231.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CCF14_RS26625	37976..38833	-	858	WP_157760420.1	incFII family plasmid replication initiator RepA	-
CCF14_RS26630	38826..38900	-	75	WP_032144212.1	RepA leader peptide Tap	-
CCF14_RS26640	39146..39394	-	249	WP_099588233.1	replication regulatory protein RepA	-
CCF14_RS26645	39465..41035	-	1571	Protein_32	IS66 family transposase	-
CCF14_RS26650	41055..41402	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeI / faeH / faeF / faeE / faeD / faeC / vat / papD / papC	1..113727	113727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-73)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11376.31 Da
• Isoelectric Point: 10.5969

>T87559 WP_099588230.1 NZ_CP024286:c36813-36523 [Escherichia albertii]
MTFNIDFDERAFKEWKTLDKTIRDQFKKKLRKLQHNPYTGAARLHGDLAGCFKIKLRASGFRLIYKVIDEEIVIWVIAVG
KRERSNVYNIASERMK

Nucleotide

• Length: 291 bp

>T87559 NZ_CP024286:c36813-36523 [Escherichia albertii]
ATGACCTTTAATATCGATTTCGACGAAAGGGCCTTTAAAGAGTGGAAGACGCTGGACAAGACAATACGTGACCAGTTTAA
AAAGAAACTCAGGAAACTACAACATAATCCCTATACTGGTGCTGCCAGGCTTCATGGTGACCTGGCTGGATGTTTTAAAA
TAAAACTAAGAGCATCCGGGTTTCGGCTTATTTATAAGGTCATTGATGAAGAAATTGTTATATGGGTGATTGCTGTGGGT
AAACGAGAGCGGAGTAACGTTTATAATATCGCCAGTGAGCGAATGAAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9855.25 Da
• Isoelectric Point: 4.5498

>AT87559 WP_099588231.1 NZ_CP024286:c37057-36803 [Escherichia albertii]
MSTVQIRVDEELKKNAWLVFEKLNLSPSEALRLFLRYVAENEKLPFSEVSVVVSDNDEDEDILNIVRERLKNPARRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT87559 NZ_CP024286:c37057-36803 [Escherichia albertii]
ATGAGTACTGTTCAAATTCGTGTAGATGAAGAACTGAAGAAAAATGCATGGCTGGTCTTCGAAAAGCTCAATCTTTCACC
TTCAGAAGCTCTCCGCCTTTTTCTCCGTTATGTCGCTGAAAATGAAAAACTTCCTTTCAGCGAAGTGTCTGTTGTGGTCA
GTGACAATGATGAGGATGAAGATATTCTGAATATTGTCAGAGAACGGCTGAAAAATCCTGCCAGACGCATCAGGGTGAAT
ATTGATGACCTTTAA