Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 84575..85122 Replicon plasmid unnamed3
Accession NZ_CP024285
Organism Escherichia albertii strain 2014C-4356

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag CCF14_RS26155 Protein ID WP_000969996.1
Coordinates 84575..84856 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag CCF14_RS26160 Protein ID WP_000079941.1
Coordinates 84853..85122 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCF14_RS26120 79586..79828 + 243 WP_000844627.1 transposase -
CCF14_RS26125 79860..80510 - 651 WP_000164043.1 tetracycline resistance transcriptional repressor TetR(A) -
CCF14_RS26130 80616..81815 + 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
CCF14_RS26135 81847..82707 - 861 WP_000493383.1 EamA family transporter -
CCF14_RS26140 82716..83369 - 654 Protein_98 helix-turn-helix domain-containing protein -
CCF14_RS28545 83325..83450 + 126 Protein_99 MerR family transcriptional regulator -
CCF14_RS26150 83687..84535 - 849 WP_001057996.1 3'-5' exonuclease -
CCF14_RS26155 84575..84856 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CCF14_RS26160 84853..85122 - 270 WP_000079941.1 hypothetical protein Antitoxin
CCF14_RS28550 85177..85335 - 159 WP_000841008.1 hypothetical protein -
CCF14_RS26175 86031..87062 - 1032 WP_000907875.1 replication initiation protein -
CCF14_RS26190 88035..88298 + 264 WP_001324596.1 ash family protein -
CCF14_RS26195 88216..88503 + 288 WP_000074849.1 conjugal transfer protein TraA -
CCF14_RS26205 88945..89478 + 534 WP_001303319.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / aac(3)-VIa / qacE / sul1 / tet(A) htpB 1..127606 127606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T87558 WP_000969996.1 NZ_CP024285:c84856-84575 [Escherichia albertii]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T87558 NZ_CP024285:c84856-84575 [Escherichia albertii]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT87558 WP_000079941.1 NZ_CP024285:c85122-84853 [Escherichia albertii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT87558 NZ_CP024285:c85122-84853 [Escherichia albertii]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References