Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 44987..45512 Replicon plasmid unnamed3
Accession NZ_CP024285
Organism Escherichia albertii strain 2014C-4356

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CCF14_RS25890 Protein ID WP_001159871.1
Coordinates 44987..45292 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag CCF14_RS25895 Protein ID WP_000813639.1
Coordinates 45294..45512 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCF14_RS25860 40983..41156 + 174 Protein_51 DUF4113 domain-containing protein -
CCF14_RS25865 41158..41574 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
CCF14_RS25870 41567..42547 - 981 WP_000688514.1 plasmid segregation protein ParM -
CCF14_RS25875 42961..43269 - 309 WP_000030199.1 hypothetical protein -
CCF14_RS25880 43356..44000 - 645 WP_001144036.1 ParA family protein -
CCF14_RS25885 44180..44986 - 807 WP_033904598.1 site-specific integrase -
CCF14_RS25890 44987..45292 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CCF14_RS25895 45294..45512 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CCF14_RS25905 46158..46418 + 261 WP_000343091.1 hypothetical protein -
CCF14_RS25910 46415..47005 + 591 WP_000194555.1 hypothetical protein -
CCF14_RS25915 47023..47370 - 348 WP_000142424.1 hypothetical protein -
CCF14_RS25920 47489..47836 + 348 WP_000762580.1 hypothetical protein -
CCF14_RS25925 47854..49734 - 1881 WP_001283356.1 colicin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / aac(3)-VIa / qacE / sul1 / tet(A) htpB 1..127606 127606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87557 WP_001159871.1 NZ_CP024285:c45292-44987 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87557 NZ_CP024285:c45292-44987 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATTCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT87557 WP_000813639.1 NZ_CP024285:c45512-45294 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87557 NZ_CP024285:c45512-45294 [Escherichia albertii]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAATGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References