Detailed information of TA system TA087548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 18331..18761
• Replicon: plasmid unnamed2
• Accession: NZ_CP024284
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CCF14_RS28300
• Protein ID: WP_114142875.1
• Coordinates: 18597..18761 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: CCF14_RS25260
• Protein ID: WP_000079928.1
• Coordinates: 18331..18600 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS25220	13678..13890	+	213	WP_001385659.1	hypothetical protein	-
CCF14_RS25225	14177..14854	+	678	WP_000392235.1	hypothetical protein	-
CCF14_RS25230	15174..15764	+	591	WP_024228807.1	DUF2726 domain-containing protein	-
CCF14_RS25235	16004..16258	+	255	WP_000083830.1	replication regulatory protein RepA	-
CCF14_RS25245	16494..16568	+	75	WP_001365705.1	RepA leader peptide Tap	-
CCF14_RS25250	16561..17418	+	858	WP_000131006.1	incFII family plasmid replication initiator RepA	-
CCF14_RS28490	18118..18276	+	159	WP_000841008.1	hypothetical protein	-
CCF14_RS25260	18331..18600	+	270	WP_000079928.1	hypothetical protein	Antitoxin
CCF14_RS28300	18597..18761	+	165	WP_114142875.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCF14_RS25265	18809..19732	+	924	WP_069723218.1	hypothetical protein	-
CCF14_RS25275	20352..20687	-	336	WP_001340323.1	hypothetical protein	-
CCF14_RS25280	21025..22005	+	981	WP_001218107.1	plasmid segregation protein ParM	-
CCF14_RS25285	22008..22430	+	423	WP_000774832.1	plasmid partitioning/stability family protein	-
CCF14_RS25295	22855..23757	+	903	WP_089581230.1	DUF1281 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..59626	59626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6622.77 Da
• Isoelectric Point: 7.1989

>T87548 WP_114142875.1 NZ_CP024284:18597-18761 [Escherichia albertii]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T87548 NZ_CP024284:18597-18761 [Escherichia albertii]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT87548 WP_000079928.1 NZ_CP024284:18331-18600 [Escherichia albertii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT87548 NZ_CP024284:18331-18600 [Escherichia albertii]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5W2J4D0