Detailed information of TA system TA087546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 17118..17610
• Replicon: plasmid unnamed1
• Accession: NZ_CP024283
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: -
• Locus tag: CCF14_RS25020
• Protein ID: WP_099588183.1
• Coordinates: 17118..17378 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: CCF14_RS25025
• Protein ID: WP_099588184.1
• Coordinates: 17368..17610 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS24975	12424..12657	+	234	WP_099588180.1	EexN family lipoprotein	-
CCF14_RS28485	12699..12986	-	288	WP_198406518.1	helix-turn-helix transcriptional regulator	-
CCF14_RS24985	13178..13516	+	339	WP_099588181.1	helix-turn-helix transcriptional regulator	-
CCF14_RS24990	13843..14835	-	993	WP_099588199.1	replication initiation protein	-
CCF14_RS25005	16192..16515	+	324	WP_000432876.1	hypothetical protein	-
CCF14_RS25010	16556..16744	+	189	WP_099588182.1	hypothetical protein	-
CCF14_RS25015	16842..17066	+	225	WP_000814384.1	hypothetical protein	-
CCF14_RS25020	17118..17378	+	261	WP_099588183.1	hypothetical protein	Toxin
CCF14_RS25025	17368..17610	+	243	WP_099588184.1	hypothetical protein	Antitoxin
CCF14_RS25030	17621..17908	+	288	WP_099588185.1	hypothetical protein	-
CCF14_RS25040	18333..18662	+	330	WP_000449469.1	hypothetical protein	-
CCF14_RS25045	18720..18998	+	279	WP_080171973.1	cobalamin biosynthesis protein CbiX	-
CCF14_RS25055	19438..19620	+	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
CCF14_RS25060	19645..20082	+	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
CCF14_RS25065	20111..20815	-	705	WP_001279867.1	hypothetical protein	-
CCF14_RS25070	20812..21573	-	762	WP_001097937.1	conjugal transfer protein TraL	-
CCF14_RS25075	21583..21990	-	408	WP_001190619.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40461	40461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9803.18 Da
• Isoelectric Point: 9.5408

>T87546 WP_099588183.1 NZ_CP024283:17118-17378 [Escherichia albertii]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEGAFDTIDFASNGLRENDKEIVSMMIQEMS
EEKNAN

Nucleotide

• Length: 261 bp

>T87546 NZ_CP024283:17118-17378 [Escherichia albertii]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGGGGCGT
TCGACACTATAGACTTTGCCAGCAACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGC
GAGGAAAAAAATGCTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9025.39 Da
• Isoelectric Point: 9.2517

>AT87546 WP_099588184.1 NZ_CP024283:17368-17610 [Escherichia albertii]
MLTENTPENIKRLRKKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTAIEFKFLLLLAGEHPDYVLCKRNSDSGSN

Nucleotide

• Length: 243 bp

>AT87546 NZ_CP024283:17368-17610 [Escherichia albertii]
ATGCTAACTGAAAATACACCAGAAAACATTAAGCGTCTGCGGAAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACATGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTGACAGCAATAG
AGTTTAAATTTCTTCTTCTTTTAGCTGGAGAACACCCTGATTATGTGCTTTGTAAAAGGAATAGCGATTCTGGAAGTAAT
TAA