Detailed information of TA system TA087524

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 804351..805058
• Replicon: chromosome
• Accession: NZ_CP024282
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A4T3Q6D2
• Locus tag: CCF14_RS04105
• Protein ID: WP_001683515.1
• Coordinates: 804351..804692 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: CCF14_RS04110
• Protein ID: WP_000939437.1
• Coordinates: 804723..805058 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS04075	800211..800768	-	558	WP_000955766.1	hypothetical protein	-
CCF14_RS04080	801162..801623	-	462	Protein_752	ATP-dependent helicase	-
CCF14_RS04085	801888..802685	-	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
CCF14_RS04090	802800..803075	-	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CCF14_RS04095	803079..803327	-	249	WP_000535212.1	ribbon-helix-helix domain-containing protein	-
CCF14_RS04100	803403..804236	-	834	WP_059258003.1	DUF4942 domain-containing protein	-
CCF14_RS04105	804351..804692	-	342	WP_001683515.1	TA system toxin CbtA family protein	Toxin
CCF14_RS04110	804723..805058	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCF14_RS04115	805058..805531	-	474	WP_001296921.1	DNA repair protein RadC	-
CCF14_RS04120	805561..806379	-	819	WP_059258004.1	DUF945 domain-containing protein	-
CCF14_RS04125	806616..807569	-	954	WP_024223205.1	hypothetical protein	-
CCF14_RS04130	808162..808776	+	615	WP_000772911.1	inovirus Gp2 family protein	-
CCF14_RS04135	808894..809112	+	219	WP_059258006.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / nleE / nleB1	790894..813342	22448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13034.05 Da
• Isoelectric Point: 9.5454

>T87524 WP_001683515.1 NZ_CP024282:c804692-804351 [Escherichia albertii]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T87524 NZ_CP024282:c804692-804351 [Escherichia albertii]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGACTGAACCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGACAGGCTACAGGTCTGCTGCGCCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT87524 WP_000939437.1 NZ_CP024282:c805058-804723 [Escherichia albertii]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT87524 NZ_CP024282:c805058-804723 [Escherichia albertii]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCTCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A4T3Q6D2

Antitoxin

• AlphaFold DB: A0A3L1KVZ6