Detailed information of TA system TA087512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 43666..44225
• Replicon: plasmid unnamed2
• Accession: NZ_CP024280
• Organism: Escherichia coli strain ATCC 43896

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: CS031_RS26920
• Protein ID: WP_000421265.1
• Coordinates: 43950..44225 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: CS031_RS26915
• Protein ID: WP_001743519.1
• Coordinates: 43666..43950 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS031_RS26890	39079..39645	+	567	WP_000904908.1	recombinase family protein	-
CS031_RS26895	39857..40054	+	198	WP_001381161.1	hypothetical protein	-
CS031_RS26900	40503..41200	+	698	Protein_44	IS1 family transposase	-
CS031_RS26905	41193..42912	+	1720	Protein_45	AAA family ATPase	-
CS031_RS26915	43666..43950	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
CS031_RS26920	43950..44225	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CS031_RS26925	44388..45464	+	1077	WP_000610005.1	hypothetical protein	-
CS031_RS26935	45816..46700	-	885	Protein_49	IS3-like element ISEc52 family transposase	-
CS031_RS26940	46710..47861	-	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
CS031_RS26945	47929..48186	-	258	Protein_51	transposase	-
CS031_RS26955	48257..48472	+	216	Protein_52	hypothetical protein	-
CS031_RS27945	49003..49077	-	75	Protein_53	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52655	52655
	-	inside	IScluster/Tn	-	-	35899..48186	12287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T87512 WP_000421265.1 NZ_CP024280:43950-44225 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T87512 NZ_CP024280:43950-44225 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT87512 WP_001743519.1 NZ_CP024280:43666-43950 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT87512 NZ_CP024280:43666-43950 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1