Detailed information of TA system TA087511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 2727..3205
• Replicon: plasmid unnamed2
• Accession: NZ_CP024280
• Organism: Escherichia coli strain ATCC 43896

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CS031_RS26680
• Protein ID: WP_000969994.1
• Coordinates: 2993..3205 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E3PPA5
• Locus tag: CS031_RS26675
• Protein ID: WP_000079921.1
• Coordinates: 2727..2996 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS031_RS26645	1..129	+	129	Protein_0	Hok/Gef family protein	-
CS031_RS26650	413..673	+	261	WP_000083816.1	replication regulatory protein RepA	-
CS031_RS26660	899..973	+	75	WP_001365705.1	RepA leader peptide Tap	-
CS031_RS26665	966..1823	+	858	WP_000130956.1	incFII family plasmid replication initiator RepA	-
CS031_RS26675	2727..2996	+	270	WP_000079921.1	hypothetical protein	Antitoxin
CS031_RS26680	2993..3205	+	213	WP_000969994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CS031_RS26690	3386..3589	-	204	Protein_6	IS91 family transposase	-
CS031_RS26695	3868..5076	-	1209	WP_001695118.1	IS91 family transposase	-
CS031_RS26700	5057..5329	-	273	WP_001695087.1	hypothetical protein	-
CS031_RS28150	5323..5412	+	90	Protein_9	invasion protein	-
CS031_RS28155	5407..5637	+	231	Protein_10	transposase	-
CS031_RS26710	5759..6972	+	1214	WP_164544955.1	IS3 family transposase	-
CS031_RS26715	7143..7543	+	401	Protein_12	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52655	52655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8175.38 Da
• Isoelectric Point: 8.5821

>T87511 WP_000969994.1 NZ_CP024280:2993-3205 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVHR

Nucleotide

• Length: 213 bp

>T87511 NZ_CP024280:2993-3205 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCATCGATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10224.63 Da
• Isoelectric Point: 9.2950

>AT87511 WP_000079921.1 NZ_CP024280:2727-2996 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKNMDRNGSQLIRDFMRQTVERQHNTWFRNQVEAGRQQLKCGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT87511 NZ_CP024280:2727-2996 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAAGATGCTTTTATGGCCGCTGCAAAAAACATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTAACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: E3PPA5