Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4867012..4867643 Replicon chromosome
Accession NZ_CP024278
Organism Escherichia coli strain ATCC 43896

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CS031_RS25230 Protein ID WP_001259385.1
Coordinates 4867012..4867287 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag CS031_RS25235 Protein ID WP_000593554.1
Coordinates 4867284..4867643 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS031_RS25215 4862016..4863083 + 1068 WP_000361467.1 HlyD family secretion protein -
CS031_RS25220 4863080..4865815 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
CS031_RS25225 4865815..4866939 + 1125 WP_001314210.1 ABC transporter permease -
CS031_RS25230 4867012..4867287 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CS031_RS25235 4867284..4867643 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CS031_RS25240 4867693..4868553 - 861 WP_001131783.1 class II aldolase -
CS031_RS25245 4868585..4868854 - 270 WP_000933060.1 HPr family phosphocarrier protein -
CS031_RS25250 4868844..4870352 - 1509 WP_001298718.1 carbohydrate kinase -
CS031_RS25255 4870345..4871703 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
CS031_RS25260 4871780..4872061 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
CS031_RS25265 4872058..4872531 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4851950..4867643 15693


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T87510 WP_001259385.1 NZ_CP024278:4867012-4867287 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T87510 NZ_CP024278:4867012-4867287 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT87510 WP_000593554.1 NZ_CP024278:4867284-4867643 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT87510 NZ_CP024278:4867284-4867643 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References