Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 85297..85822 Replicon plasmid unnamed2
Accession NZ_CP024277
Organism Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag COG40_RS25785 Protein ID WP_001159871.1
Coordinates 85297..85602 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag COG40_RS25790 Protein ID WP_000829078.1
Coordinates 85604..85822 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG40_RS25760 80451..81580 + 1130 Protein_96 IS110 family transposase -
COG40_RS25765 81833..83063 - 1231 Protein_97 IS66 family transposase -
COG40_RS25770 83273..83581 - 309 WP_000030204.1 molecular chaperone GroEL -
COG40_RS25775 83668..84312 - 645 WP_001144037.1 ParA family protein -
COG40_RS25780 84490..85296 - 807 WP_000016960.1 site-specific integrase -
COG40_RS25785 85297..85602 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
COG40_RS25790 85604..85822 - 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
COG40_RS25805 86344..86601 + 258 WP_000343095.1 hypothetical protein -
COG40_RS25810 86601..87143 + 543 WP_000194544.1 hypothetical protein -
COG40_RS25815 87387..88229 + 843 WP_162749921.1 polysaccharide deacetylase family protein -
COG40_RS25820 88232..89320 + 1089 WP_099590715.1 putative hexosyltransferase CapU -
COG40_RS25825 89325..90275 + 951 WP_001398306.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - eltB / eltA / etpA / etpB / cs3 1..100184 100184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87487 WP_001159871.1 NZ_CP024277:c85602-85297 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87487 NZ_CP024277:c85602-85297 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT87487 WP_000829078.1 NZ_CP024277:c85822-85604 [Escherichia coli O6:H16]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87487 NZ_CP024277:c85822-85604 [Escherichia coli O6:H16]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References