Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33700..34225 Replicon plasmid unnamed1
Accession NZ_CP024276
Organism Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag COG40_RS25180 Protein ID WP_001159871.1
Coordinates 33700..34005 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag COG40_RS25185 Protein ID WP_000813634.1
Coordinates 34007..34225 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG40_RS25155 29944..30647 - 704 Protein_37 helix-turn-helix domain-containing protein -
COG40_RS25160 30895..31575 + 681 WP_000452091.1 EAL domain-containing protein -
COG40_RS25170 32015..32656 - 642 WP_001705742.1 recombinase family protein -
COG40_RS25175 32986..33699 - 714 WP_000016957.1 site-specific integrase -
COG40_RS25180 33700..34005 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
COG40_RS25185 34007..34225 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
COG40_RS25195 34670..34927 + 258 WP_000343092.1 hypothetical protein -
COG40_RS25200 34927..35457 + 531 WP_011387704.1 hypothetical protein -
COG40_RS25205 35701..36543 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
COG40_RS25210 36546..37634 + 1089 Protein_46 putative hexosyltransferase CapU -
COG40_RS25215 37639..38177 + 539 Protein_47 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..38177 38177
- flank IS/Tn - - 32015..32638 623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87486 WP_001159871.1 NZ_CP024276:c34005-33700 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87486 NZ_CP024276:c34005-33700 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT87486 WP_000813634.1 NZ_CP024276:c34225-34007 [Escherichia coli O6:H16]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87486 NZ_CP024276:c34225-34007 [Escherichia coli O6:H16]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References