Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4582547..4583044 Replicon chromosome
Accession NZ_CP024275
Organism Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag COG40_RS23830 Protein ID WP_001173343.1
Coordinates 4582871..4583044 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag COG40_RS23825 Protein ID WP_071597404.1
Coordinates 4582547..4582843 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG40_RS23800 4579278..4579997 - 720 WP_000611288.1 two-component system response regulator DcuR -
COG40_RS23805 4579994..4581625 - 1632 WP_001216490.1 sensor histidine kinase -
COG40_RS23810 4581806..4582036 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
COG40_RS23815 4582048..4582320 + 273 WP_000405647.1 N-acetyltransferase -
COG40_RS23825 4582547..4582843 + 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
COG40_RS23830 4582871..4583044 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
COG40_RS23835 4583168..4584685 - 1518 WP_001295074.1 lysine--tRNA ligase -
COG40_RS23840 4584922..4586379 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87485 WP_001173343.1 NZ_CP024275:4582871-4583044 [Escherichia coli O6:H16]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87485 NZ_CP024275:4582871-4583044 [Escherichia coli O6:H16]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT87485 WP_071597404.1 NZ_CP024275:4582547-4582843 [Escherichia coli O6:H16]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87485 NZ_CP024275:4582547-4582843 [Escherichia coli O6:H16]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References