Detailed information of TA system TA087484

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4070477..4071312
• Replicon: chromosome
• Accession: NZ_CP024275
• Organism: Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: COG40_RS21265
• Protein ID: WP_000854743.1
• Coordinates: 4070935..4071312 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: COG40_RS21260
• Protein ID: WP_001285298.1
• Coordinates: 4070477..4070845 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG40_RS21235	4067591..4068412	+	822	WP_001706654.1	DUF945 domain-containing protein	-
COG40_RS21240	4068504..4068989	+	486	WP_000206659.1	antirestriction protein	-
COG40_RS21245	4069004..4069480	+	477	WP_001186774.1	RadC family protein	-
COG40_RS21250	4069543..4069764	+	222	WP_000692307.1	DUF987 domain-containing protein	-
COG40_RS21255	4069783..4070427	+	645	WP_000086754.1	hypothetical protein	-
COG40_RS21260	4070477..4070845	+	369	WP_001285298.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
COG40_RS21265	4070935..4071312	+	378	WP_000854743.1	TA system toxin CbtA family protein	Toxin
COG40_RS21270	4071309..4071797	+	489	WP_000761693.1	hypothetical protein	-
COG40_RS21275	4071809..4072006	+	198	WP_000839275.1	DUF957 domain-containing protein	-
COG40_RS21280	4072103..4072941	+	839	Protein_3905	DUF4942 domain-containing protein	-
COG40_RS21295	4073738..4074922	+	1185	WP_001172882.1	sugar efflux transporter	-
COG40_RS21300	4075033..4075956	+	924	WP_000535961.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14172.08 Da
• Isoelectric Point: 6.8519

>T87484 WP_000854743.1 NZ_CP024275:4070935-4071312 [Escherichia coli O6:H16]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDSVNFLVEKYALVRTDQPGF
GACTYSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T87484 NZ_CP024275:4070935-4071312 [Escherichia coli O6:H16]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATTCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
GGTGCCTGTACCTATTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13710.44 Da
• Isoelectric Point: 5.8799

>AT87484 WP_001285298.1 NZ_CP024275:4070477-4070845 [Escherichia coli O6:H16]
MSDSRHETNYPDDHNDRPWWGLPCTVIPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKN

Nucleotide

• Length: 369 bp

>AT87484 NZ_CP024275:4070477-4070845 [Escherichia coli O6:H16]
ATGTCAGACTCGCGCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAT
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGCAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAATTAA