Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3156661..3157315 Replicon chromosome
Accession NZ_CP024275
Organism Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag COG40_RS16335 Protein ID WP_000244781.1
Coordinates 3156661..3157068 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag COG40_RS16340 Protein ID WP_000354046.1
Coordinates 3157049..3157315 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG40_RS16315 3152618..3154351 - 1734 WP_001394741.1 single-stranded-DNA-specific exonuclease RecJ -
COG40_RS16320 3154357..3155067 - 711 WP_000715224.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
COG40_RS16325 3155092..3155988 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
COG40_RS16330 3156100..3156621 + 522 WP_001055874.1 flavodoxin FldB -
COG40_RS16335 3156661..3157068 - 408 WP_000244781.1 protein YgfX Toxin
COG40_RS16340 3157049..3157315 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
COG40_RS16345 3157558..3158538 + 981 WP_000886087.1 tRNA-modifying protein YgfZ -
COG40_RS16350 3158615..3159274 - 660 WP_047608329.1 hemolysin III family protein -
COG40_RS16355 3159438..3159749 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
COG40_RS16360 3159794..3161227 + 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T87480 WP_000244781.1 NZ_CP024275:c3157068-3156661 [Escherichia coli O6:H16]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87480 NZ_CP024275:c3157068-3156661 [Escherichia coli O6:H16]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87480 WP_000354046.1 NZ_CP024275:c3157315-3157049 [Escherichia coli O6:H16]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87480 NZ_CP024275:c3157315-3157049 [Escherichia coli O6:H16]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References