Detailed information of TA system TA087469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1561190..1561828
• Replicon: chromosome
• Accession: NZ_CP024275
• Organism: Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: COG40_RS08160
• Protein ID: WP_000813794.1
• Coordinates: 1561190..1561366 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: COG40_RS08165
• Protein ID: WP_001270286.1
• Coordinates: 1561412..1561828 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG40_RS08140	1556809..1558023	-	1215	WP_071597387.1	BenE family transporter YdcO	-
COG40_RS08145	1558076..1558612	+	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
COG40_RS08150	1558685..1560646	+	1962	WP_001355676.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
COG40_RS08155	1560738..1560968	-	231	WP_000494244.1	YncJ family protein	-
COG40_RS08160	1561190..1561366	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
COG40_RS08165	1561412..1561828	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
COG40_RS08170	1561907..1563313	+	1407	WP_000760586.1	PLP-dependent aminotransferase family protein	-
COG40_RS08175	1563558..1564703	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
COG40_RS08180	1564721..1565734	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
COG40_RS08185	1565735..1566676	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1555504..1556769	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T87469 WP_000813794.1 NZ_CP024275:1561190-1561366 [Escherichia coli O6:H16]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T87469 NZ_CP024275:1561190-1561366 [Escherichia coli O6:H16]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87469 WP_001270286.1 NZ_CP024275:1561412-1561828 [Escherichia coli O6:H16]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87469 NZ_CP024275:1561412-1561828 [Escherichia coli O6:H16]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB