Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 68893..69467 Replicon plasmid unnamed
Accession NZ_CP024274
Organism Escherichia coli strain F9792

Toxin (Protein)


Gene name relE Uniprot ID A0A0S3PMZ8
Locus tag A5T63_RS25390 Protein ID WP_001595119.1
Coordinates 69141..69467 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0S3PN98
Locus tag A5T63_RS25385 Protein ID WP_001595120.1
Coordinates 68893..69144 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5T63_RS25355 63950..64372 + 423 WP_001595126.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
A5T63_RS25360 64372..65646 + 1275 WP_001595125.1 Y-family DNA polymerase -
A5T63_RS26385 65685..66008 - 324 WP_000143877.1 hypothetical protein -
A5T63_RS25370 66005..66418 - 414 WP_032159708.1 hypothetical protein -
A5T63_RS25375 66442..67473 - 1032 WP_001595123.1 ParB/RepB/Spo0J family partition protein -
A5T63_RS25380 67473..68339 - 867 WP_001595122.1 AAA family ATPase -
A5T63_RS25385 68893..69144 + 252 WP_001595120.1 hypothetical protein Antitoxin
A5T63_RS25390 69141..69467 + 327 WP_001595119.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A5T63_RS25395 69571..69867 + 297 WP_000766063.1 hypothetical protein -
A5T63_RS25400 69867..71044 + 1178 Protein_87 IS91 family transposase -
A5T63_RS25415 71917..72894 + 978 WP_023486339.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - estIa / cssA / cofA / faeD / faeE 1..145089 145089


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12187.44 Da        Isoelectric Point: 10.4373

>T87460 WP_001595119.1 NZ_CP024274:69141-69467 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKAIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAIGK
RERSDVYNLASRVGSLAAAACHGLKLPL

Download         Length: 327 bp

>T87460 NZ_CP024274:69141-69467 [Escherichia coli]
ATGACTTACACAGTCAAATTCAGGGATGATGCACTGAAAGAATGGCTGAAACTGGATAAGGCCATTCAACAGCAATTTGC
TAAAAAGCTAAAGAAGTGCAGTGAAAATCCTCATATTCCCTCTGCTAAGCTGCGCGGGATAAAAGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATAGATGATCAATTAATTATCGCTGTTGTTGCTATTGGGAAA
CGTGAACGTAGTGATGTTTATAATCTTGCCAGCCGAGTAGGCAGCCTGGCGGCTGCGGCTTGTCATGGACTGAAATTACC
GTTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9144.43 Da        Isoelectric Point: 4.2975

>AT87460 WP_001595120.1 NZ_CP024274:68893-69144 [Escherichia coli]
MPNIILTDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYGKMLDAMDDQELVKLVAERSNQKLHDVDLD
SYL

Download         Length: 252 bp

>AT87460 NZ_CP024274:68893-69144 [Escherichia coli]
ATGCCTAACATCATTCTTACCGACACAAGCGCCAGTGTAAGCGAACTTAAGAAAAACCCGATGGCTACAGTCAGCGCTGG
TGATGGCTACCCAGTTGCTATTCTGAACCGCAACCAGCCAGCCTTTTACTGCGTACCCGCAGAGTTGTACGGAAAGATGC
TCGATGCCATGGACGATCAGGAACTGGTTAAGCTAGTCGCCGAACGCAGCAACCAGAAGCTGCACGATGTGGATCTGGAT
AGCTACTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S3PMZ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3PN98

References