Detailed information of TA system TA087444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1545630..1546268
• Replicon: chromosome
• Accession: NZ_CP024273
• Organism: Escherichia coli strain F9792

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T4J978
• Locus tag: A5T63_RS08145
• Protein ID: WP_001612987.1
• Coordinates: 1545630..1545806 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A5T63_RS08150
• Protein ID: WP_001270286.1
• Coordinates: 1545852..1546268 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5T63_RS08125	1541249..1542463	-	1215	WP_072148478.1	BenE family transporter YdcO	-
A5T63_RS08130	1542516..1543052	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
A5T63_RS08135	1543125..1545086	+	1962	WP_001718255.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A5T63_RS08140	1545178..1545408	-	231	WP_000494244.1	YncJ family protein	-
A5T63_RS08145	1545630..1545806	+	177	WP_001612987.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A5T63_RS08150	1545852..1546268	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A5T63_RS08155	1546347..1547753	+	1407	WP_001718258.1	PLP-dependent aminotransferase family protein	-
A5T63_RS08160	1547998..1549143	+	1146	WP_001612991.1	ABC transporter substrate-binding protein	-
A5T63_RS08165	1549161..1550174	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
A5T63_RS08170	1550175..1551116	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1540061..1541209	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6725.78 Da
• Isoelectric Point: 10.9223

>T87444 WP_001612987.1 NZ_CP024273:1545630-1545806 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKVRFHGMRSVMPRHPCDEIKEPLRKAIQKQLGLS

Nucleotide

• Length: 177 bp

>T87444 NZ_CP024273:1545630-1545806 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAAGTCAG
GTTTCATGGGATGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCAGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87444 WP_001270286.1 NZ_CP024273:1545852-1546268 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87444 NZ_CP024273:1545852-1546268 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGAGAATTGGCAAACCTAAACAGGAAAT
TACTCGTTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A3T4J978