Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4009033..4009651 Replicon chromosome
Accession NZ_CP024269
Organism Escherichia coli strain F8111-1SC3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CCV27_RS20220 Protein ID WP_001291435.1
Coordinates 4009033..4009251 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CCV27_RS20225 Protein ID WP_000344800.1
Coordinates 4009277..4009651 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCV27_RS20185 4004322..4004894 + 573 WP_000779826.1 lipoprotein -
CCV27_RS20190 4004925..4005236 - 312 WP_000409911.1 MGMT family protein -
CCV27_RS20200 4005615..4005968 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CCV27_RS20205 4006010..4007560 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CCV27_RS20210 4007724..4008194 - 471 WP_000136192.1 YlaC family protein -
CCV27_RS20215 4008310..4008861 - 552 WP_099492730.1 maltose O-acetyltransferase -
CCV27_RS20220 4009033..4009251 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CCV27_RS20225 4009277..4009651 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CCV27_RS20230 4010197..4013346 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CCV27_RS20235 4013369..4014562 - 1194 WP_024171442.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87435 WP_001291435.1 NZ_CP024269:c4009251-4009033 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87435 NZ_CP024269:c4009251-4009033 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87435 WP_000344800.1 NZ_CP024269:c4009651-4009277 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87435 NZ_CP024269:c4009651-4009277 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGATCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAGGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References