Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2403911..2404542 Replicon chromosome
Accession NZ_CP024269
Organism Escherichia coli strain F8111-1SC3

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CCV27_RS12380 Protein ID WP_001259385.1
Coordinates 2404267..2404542 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CCV27_RS12375 Protein ID WP_000593555.1
Coordinates 2403911..2404270 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCV27_RS12350 2400043..2400987 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
CCV27_RS12355 2400984..2401817 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CCV27_RS12360 2401817..2402581 + 765 WP_001614016.1 nickel import ATP-binding protein NikD -
CCV27_RS12365 2402578..2403384 + 807 WP_001614017.1 nickel import ATP-binding protein NikE -
CCV27_RS12370 2403390..2403791 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CCV27_RS12375 2403911..2404270 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CCV27_RS12380 2404267..2404542 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CCV27_RS12385 2404615..2405739 - 1125 WP_001314210.1 ABC transporter permease -
CCV27_RS12390 2405739..2408474 - 2736 WP_001614021.1 ribosome-associated ATPase/putative transporter RbbA -
CCV27_RS12395 2408471..2409538 - 1068 WP_001614022.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T87431 WP_001259385.1 NZ_CP024269:c2404542-2404267 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T87431 NZ_CP024269:c2404542-2404267 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT87431 WP_000593555.1 NZ_CP024269:c2404270-2403911 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT87431 NZ_CP024269:c2404270-2403911 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References