Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28067..28592 Replicon plasmid unnamed2
Accession NZ_CP024268
Organism Escherichia coli O6:H16 strain F6699

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag COG39_RS24540 Protein ID WP_001159871.1
Coordinates 28067..28372 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag COG39_RS24545 Protein ID WP_000829078.1
Coordinates 28374..28592 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG39_RS24515 (23221) 23221..24350 + 1130 Protein_27 IS110-like element ISEc45 family transposase -
COG39_RS25750 (24603) 24603..25833 - 1231 Protein_28 IS66 family transposase -
COG39_RS24525 (26043) 26043..26351 - 309 WP_000030204.1 molecular chaperone GroEL -
COG39_RS24530 (26438) 26438..27082 - 645 WP_001144037.1 ParA family protein -
COG39_RS24535 (27260) 27260..28066 - 807 WP_000016960.1 site-specific integrase -
COG39_RS24540 (28067) 28067..28372 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
COG39_RS24545 (28374) 28374..28592 - 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
COG39_RS24560 (29114) 29114..29371 + 258 WP_000343095.1 hypothetical protein -
COG39_RS24565 (29371) 29371..29892 + 522 WP_044307843.1 hypothetical protein -
COG39_RS24570 (30136) 30136..30978 + 843 WP_162749921.1 polysaccharide deacetylase family protein -
COG39_RS24575 (30981) 30981..32069 + 1089 WP_012131088.1 putative hexosyltransferase CapU -
COG39_RS24580 (32074) 32074..33024 + 951 WP_001398306.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cs3 / eltB / eltA 1..95350 95350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87414 WP_001159871.1 NZ_CP024268:c28372-28067 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87414 NZ_CP024268:c28372-28067 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT87414 WP_000829078.1 NZ_CP024268:c28592-28374 [Escherichia coli O6:H16]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87414 NZ_CP024268:c28592-28374 [Escherichia coli O6:H16]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References