Detailed information of TA system TA087413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 13251..13810
• Replicon: plasmid unnamed1
• Accession: NZ_CP024267
• Organism: Escherichia coli O6:H16 strain F6699

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: COG39_RS24195
• Protein ID: WP_000421257.1
• Coordinates: 13535..13810 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: COG39_RS24190
• Protein ID: WP_024169770.1
• Coordinates: 13251..13535 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG39_RS24160 (8657)	8657..9139	+	483	WP_001751479.1	transposase	-
COG39_RS24165 (9078)	9078..9428	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
COG39_RS24170 (9459)	9459..11072	+	1614	Protein_13	IS66-like element ISEc23 family transposase	-
COG39_RS24175 (11079)	11079..12061	+	983	Protein_14	IS110 family transposase	-
COG39_RS24190 (13251)	13251..13535	+	285	WP_024169770.1	ribbon-helix-helix domain-containing protein	Antitoxin
COG39_RS24195 (13535)	13535..13810	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
COG39_RS24200 (13916)	13916..14119	+	204	Protein_17	hypothetical protein	-
COG39_RS24205 (14649)	14649..15215	+	567	WP_134881237.1	hypothetical protein	-
COG39_RS24210 (15565)	15565..15924	-	360	Protein_19	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
COG39_RS24220 (17336)	17336..17732	-	397	Protein_20	plasmid partitioning/stability family protein	-
COG39_RS24225 (17732)	17732..18691	-	960	WP_000921959.1	plasmid segregation protein ParM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34042	34042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T87413 WP_000421257.1 NZ_CP024267:13535-13810 [Escherichia coli O6:H16]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T87413 NZ_CP024267:13535-13810 [Escherichia coli O6:H16]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10530.94 Da
• Isoelectric Point: 5.6558

>AT87413 WP_024169770.1 NZ_CP024267:13251-13535 [Escherichia coli O6:H16]
IQMKNNSAQTTKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQTW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT87413 NZ_CP024267:13251-13535 [Escherichia coli O6:H16]
ATACAAATGAAAAACAATAGTGCACAAACAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCTGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGACCTGG
GCGGACAGCCTCAGTACGGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5