Detailed information of TA system TA087410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3613031..3613669
• Replicon: chromosome
• Accession: NZ_CP024266
• Organism: Escherichia coli O6:H16 strain F6699

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: COG39_RS17705
• Protein ID: WP_000813794.1
• Coordinates: 3613493..3613669 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: COG39_RS17700
• Protein ID: WP_001270286.1
• Coordinates: 3613031..3613447 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG39_RS17680 (3608183)	3608183..3609124	-	942	WP_001251313.1	ABC transporter permease	-
COG39_RS17685 (3609125)	3609125..3610138	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
COG39_RS17690 (3610156)	3610156..3611301	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
COG39_RS17695 (3611546)	3611546..3612952	-	1407	WP_000760586.1	PLP-dependent aminotransferase family protein	-
COG39_RS17700 (3613031)	3613031..3613447	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
COG39_RS17705 (3613493)	3613493..3613669	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
COG39_RS17710 (3613891)	3613891..3614121	+	231	WP_000494244.1	YncJ family protein	-
COG39_RS17715 (3614213)	3614213..3616174	-	1962	WP_001355676.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
COG39_RS17720 (3616247)	3616247..3616783	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
COG39_RS17725 (3616875)	3616875..3618050	+	1176	WP_001418601.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3618090..3619355	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T87410 WP_000813794.1 NZ_CP024266:c3613669-3613493 [Escherichia coli O6:H16]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T87410 NZ_CP024266:c3613669-3613493 [Escherichia coli O6:H16]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87410 WP_001270286.1 NZ_CP024266:c3613447-3613031 [Escherichia coli O6:H16]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87410 NZ_CP024266:c3613447-3613031 [Escherichia coli O6:H16]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB