Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 327775..328351 Replicon chromosome
Accession NZ_CP024266
Organism Escherichia coli O6:H16 strain F6699

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag COG39_RS01545 Protein ID WP_001297643.1
Coordinates 328064..328351 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag COG39_RS01540 Protein ID WP_000063160.1
Coordinates 327775..328077 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG39_RS01525 (324371) 324371..326521 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
COG39_RS01530 (326571) 326571..326774 + 204 WP_000467859.1 YbdD/YjiX family protein -
COG39_RS01535 (326785) 326785..327741 + 957 WP_001297640.1 GTPase -
COG39_RS01540 (327775) 327775..328077 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
COG39_RS01545 (328064) 328064..328351 - 288 WP_001297643.1 BrnT family toxin Toxin
COG39_RS25795 (328550) 328550..329246 - 697 Protein_302 IS1 family transposase -
COG39_RS01555 (329561) 329561..330793 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
COG39_RS01560 (330834) 330834..332114 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 328550..328759 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T87393 WP_001297643.1 NZ_CP024266:c328351-328064 [Escherichia coli O6:H16]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T87393 NZ_CP024266:c328351-328064 [Escherichia coli O6:H16]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT87393 WP_000063160.1 NZ_CP024266:c328077-327775 [Escherichia coli O6:H16]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT87393 NZ_CP024266:c328077-327775 [Escherichia coli O6:H16]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References