Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4782993..4783611 Replicon chromosome
Accession NZ_CP024263
Organism Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag COG38_RS24555 Protein ID WP_001291435.1
Coordinates 4782993..4783211 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag COG38_RS24560 Protein ID WP_000344800.1
Coordinates 4783237..4783611 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG38_RS24520 4778282..4778854 + 573 WP_000779826.1 lipoprotein -
COG38_RS24525 4778885..4779196 - 312 WP_000409911.1 MGMT family protein -
COG38_RS24535 4779575..4779928 + 354 WP_000878140.1 DUF1428 domain-containing protein -
COG38_RS24540 4779970..4781520 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
COG38_RS24545 4781684..4782154 - 471 WP_000136192.1 YlaC family protein -
COG38_RS24550 4782270..4782821 - 552 WP_000102564.1 maltose O-acetyltransferase -
COG38_RS24555 4782993..4783211 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
COG38_RS24560 4783237..4783611 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
COG38_RS24565 4784157..4787306 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
COG38_RS24570 4787329..4788522 - 1194 WP_024171442.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87389 WP_001291435.1 NZ_CP024263:c4783211-4782993 [Escherichia coli O169:H41]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87389 NZ_CP024263:c4783211-4782993 [Escherichia coli O169:H41]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87389 WP_000344800.1 NZ_CP024263:c4783611-4783237 [Escherichia coli O169:H41]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87389 NZ_CP024263:c4783611-4783237 [Escherichia coli O169:H41]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGATCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAGGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References