Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4193794..4194370 Replicon chromosome
Accession NZ_CP024263
Organism Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag COG38_RS21475 Protein ID WP_001297643.1
Coordinates 4193794..4194081 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag COG38_RS21480 Protein ID WP_000063160.1
Coordinates 4194068..4194370 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG38_RS21455 4190032..4191312 - 1281 WP_001037390.1 DUF445 domain-containing protein -
COG38_RS21460 4191353..4192585 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
COG38_RS21470 4192899..4193595 + 697 Protein_3921 IS1 family transposase -
COG38_RS21475 4193794..4194081 + 288 WP_001297643.1 BrnT family toxin Toxin
COG38_RS21480 4194068..4194370 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
COG38_RS21485 4194404..4195360 - 957 WP_001297640.1 GTPase -
COG38_RS21490 4195371..4195574 - 204 WP_000467859.1 YbdD/YjiX family protein -
COG38_RS21495 4195624..4197774 - 2151 WP_001340937.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4193386..4193595 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T87387 WP_001297643.1 NZ_CP024263:4193794-4194081 [Escherichia coli O169:H41]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T87387 NZ_CP024263:4193794-4194081 [Escherichia coli O169:H41]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT87387 WP_000063160.1 NZ_CP024263:4194068-4194370 [Escherichia coli O169:H41]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT87387 NZ_CP024263:4194068-4194370 [Escherichia coli O169:H41]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References