Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3964886..3965383 Replicon chromosome
Accession NZ_CP024263
Organism Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag COG38_RS20205 Protein ID WP_001173343.1
Coordinates 3965210..3965383 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag COG38_RS20200 Protein ID WP_000398619.1
Coordinates 3964886..3965182 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG38_RS20175 3961617..3962336 - 720 WP_000611288.1 two-component system response regulator DcuR -
COG38_RS20180 3962333..3963964 - 1632 WP_001216477.1 sensor histidine kinase -
COG38_RS20185 3964145..3964375 + 231 WP_001612217.1 (4Fe-4S)-binding protein -
COG38_RS20190 3964387..3964659 + 273 WP_000405647.1 N-acetyltransferase -
COG38_RS20200 3964886..3965182 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
COG38_RS20205 3965210..3965383 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
COG38_RS20210 3965502..3967019 - 1518 WP_001295087.1 lysine--tRNA ligase -
COG38_RS20215 3967256..3968713 - 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87385 WP_001173343.1 NZ_CP024263:3965210-3965383 [Escherichia coli O169:H41]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87385 NZ_CP024263:3965210-3965383 [Escherichia coli O169:H41]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT87385 WP_000398619.1 NZ_CP024263:3964886-3965182 [Escherichia coli O169:H41]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87385 NZ_CP024263:3964886-3965182 [Escherichia coli O169:H41]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References