Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3130783..3131414 Replicon chromosome
Accession NZ_CP024263
Organism Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag COG38_RS16230 Protein ID WP_001259385.1
Coordinates 3131139..3131414 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag COG38_RS16225 Protein ID WP_000593555.1
Coordinates 3130783..3131142 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG38_RS16200 3126915..3127859 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
COG38_RS16205 3127856..3128689 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
COG38_RS16210 3128689..3129453 + 765 WP_001614016.1 nickel import ATP-binding protein NikD -
COG38_RS16215 3129450..3130256 + 807 WP_001614017.1 nickel import ATP-binding protein NikE -
COG38_RS16220 3130262..3130663 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
COG38_RS16225 3130783..3131142 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
COG38_RS16230 3131139..3131414 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
COG38_RS16235 3131487..3132611 - 1125 WP_001314210.1 ABC transporter permease -
COG38_RS16240 3132611..3135346 - 2736 WP_001614021.1 ribosome-associated ATPase/putative transporter RbbA -
COG38_RS16245 3135343..3136410 - 1068 WP_001614022.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T87383 WP_001259385.1 NZ_CP024263:c3131414-3131139 [Escherichia coli O169:H41]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T87383 NZ_CP024263:c3131414-3131139 [Escherichia coli O169:H41]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT87383 WP_000593555.1 NZ_CP024263:c3131142-3130783 [Escherichia coli O169:H41]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT87383 NZ_CP024263:c3131142-3130783 [Escherichia coli O169:H41]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References