Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3109276..3110076 Replicon chromosome
Accession NZ_CP024263
Organism Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4AFE4
Locus tag COG38_RS16105 Protein ID WP_001614005.1
Coordinates 3109276..3109803 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag COG38_RS16110 Protein ID WP_001277108.1
Coordinates 3109810..3110076 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG38_RS16075 3104351..3105118 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
COG38_RS16080 3105115..3106392 - 1278 WP_001614002.1 branched chain amino acid ABC transporter permease LivM -
COG38_RS16085 3106389..3107315 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
COG38_RS16090 3107363..3108472 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
COG38_RS16100 3108896..3109279 + 384 WP_001614003.1 aspartate 1-decarboxylase autocleavage activator PanM -
COG38_RS16105 3109276..3109803 - 528 WP_001614005.1 GNAT family N-acetyltransferase Toxin
COG38_RS16110 3109810..3110076 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
COG38_RS16115 3110226..3111329 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
COG38_RS16120 3111600..3112454 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
COG38_RS16125 3112699..3113757 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
COG38_RS16130 3113750..3114418 - 669 WP_001718990.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19676.62 Da        Isoelectric Point: 7.3502

>T87382 WP_001614005.1 NZ_CP024263:c3109803-3109276 [Escherichia coli O169:H41]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T87382 NZ_CP024263:c3109803-3109276 [Escherichia coli O169:H41]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT87382 WP_001277108.1 NZ_CP024263:c3110076-3109810 [Escherichia coli O169:H41]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT87382 NZ_CP024263:c3110076-3109810 [Escherichia coli O169:H41]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4AFE4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References