Detailed information of TA system TA087371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 969883..970521
• Replicon: chromosome
• Accession: NZ_CP024263
• Organism: Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T4J978
• Locus tag: COG38_RS05225
• Protein ID: WP_001612987.1
• Coordinates: 969883..970059 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: COG38_RS05230
• Protein ID: WP_001270286.1
• Coordinates: 970105..970521 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG38_RS05205	965502..966716	-	1215	WP_072148478.1	BenE family transporter YdcO	-
COG38_RS05210	966769..967305	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
COG38_RS05215	967378..969339	+	1962	WP_001718255.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
COG38_RS05220	969431..969661	-	231	WP_000494244.1	YncJ family protein	-
COG38_RS05225	969883..970059	+	177	WP_001612987.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
COG38_RS05230	970105..970521	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
COG38_RS05235	970600..972006	+	1407	WP_001718258.1	PLP-dependent aminotransferase family protein	-
COG38_RS05240	972251..973396	+	1146	WP_001612991.1	ABC transporter substrate-binding protein	-
COG38_RS05245	973414..974427	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
COG38_RS05250	974428..975369	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	964314..965462	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6725.78 Da
• Isoelectric Point: 10.9223

>T87371 WP_001612987.1 NZ_CP024263:969883-970059 [Escherichia coli O169:H41]
VKQSEFRRWLESQGVDVANGSNHLKVRFHGMRSVMPRHPCDEIKEPLRKAIQKQLGLS

Nucleotide

• Length: 177 bp

>T87371 NZ_CP024263:969883-970059 [Escherichia coli O169:H41]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAAGTCAG
GTTTCATGGGATGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCAGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87371 WP_001270286.1 NZ_CP024263:970105-970521 [Escherichia coli O169:H41]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87371 NZ_CP024263:970105-970521 [Escherichia coli O169:H41]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGAGAATTGGCAAACCTAAACAGGAAAT
TACTCGTTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A3T4J978