Detailed information of TA system TA087366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 108744..109269
• Replicon: plasmid unnamed2
• Accession: NZ_CP024262
• Organism: Escherichia coli strain F5656C1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: A4V61_RS25825
• Protein ID: WP_001159871.1
• Coordinates: 108744..109049 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: A4V61_RS25830
• Protein ID: WP_072328065.1
• Coordinates: 109051..109269 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4V61_RS25800	103898..105027	+	1130	Protein_123	IS110 family transposase	-
A4V61_RS25805	105280..106510	-	1231	Protein_124	IS66 family transposase	-
A4V61_RS25810	106720..107028	-	309	WP_000030204.1	molecular chaperone GroEL	-
A4V61_RS25815	107115..107759	-	645	WP_001144037.1	ParA family protein	-
A4V61_RS25820	107937..108743	-	807	WP_000016960.1	site-specific integrase	-
A4V61_RS25825	108744..109049	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
A4V61_RS25830	109051..109269	-	219	WP_072328065.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
A4V61_RS25845	109791..110048	+	258	WP_000343095.1	hypothetical protein	-
A4V61_RS25850	110048..110590	+	543	WP_032328642.1	hypothetical protein	-
A4V61_RS25855	110834..111676	+	843	WP_160371899.1	polysaccharide deacetylase family protein	-
A4V61_RS25860	111679..112767	+	1089	WP_064734538.1	putative hexosyltransferase CapU	-
A4V61_RS25865	112772..113722	+	951	WP_001699589.1	virulence factor VirK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltB / eltA / etpB / cs3	1..119846	119846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T87366 WP_001159871.1 NZ_CP024262:c109049-108744 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T87366 NZ_CP024262:c109049-108744 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8470.54 Da
• Isoelectric Point: 6.3212

>AT87366 WP_072328065.1 NZ_CP024262:c109269-109051 [Escherichia coli]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVTRFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT87366 NZ_CP024262:c109269-109051 [Escherichia coli]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCACCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8