Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15844..16369 Replicon plasmid unnamed2
Accession NZ_CP024262
Organism Escherichia coli strain F5656C1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag A4V61_RS25175 Protein ID WP_001159871.1
Coordinates 15844..16149 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A4V61_RS25180 Protein ID WP_000813634.1
Coordinates 16151..16369 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4V61_RS25150 12089..12792 - 704 Protein_11 helix-turn-helix domain-containing protein -
A4V61_RS25155 13040..13719 + 680 Protein_12 EAL domain-containing protein -
A4V61_RS25165 14159..14800 - 642 WP_001705742.1 recombinase family protein -
A4V61_RS25170 15130..15843 - 714 WP_000016957.1 site-specific integrase -
A4V61_RS25175 15844..16149 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
A4V61_RS25180 16151..16369 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A4V61_RS25190 16814..17071 + 258 WP_000343092.1 hypothetical protein -
A4V61_RS25195 17071..17601 + 531 WP_011387704.1 hypothetical protein -
A4V61_RS25200 17845..18687 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
A4V61_RS25205 18690..19778 + 1089 WP_064734538.1 putative hexosyltransferase CapU -
A4V61_RS25210 19783..20733 + 951 WP_001699589.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - eltB / eltA / etpB / cs3 1..119846 119846
- flank IS/Tn - - 14159..14800 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87365 WP_001159871.1 NZ_CP024262:c16149-15844 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87365 NZ_CP024262:c16149-15844 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT87365 WP_000813634.1 NZ_CP024262:c16369-16151 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87365 NZ_CP024262:c16369-16151 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References