Detailed information of TA system TA087364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 29877..30399
• Replicon: plasmid unnamed1
• Accession: NZ_CP024261
• Organism: Escherichia coli strain F5656C1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A4V61_RS24995
• Protein ID: WP_000220563.1
• Coordinates: 30118..30399 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: A4V61_RS24990
• Protein ID: WP_000121743.1
• Coordinates: 29877..30128 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4V61_RS24960	24946..25617	+	672	WP_099524366.1	P-loop NTPase	-
A4V61_RS24965	25715..25996	+	282	WP_001185482.1	hypothetical protein	-
A4V61_RS24970	26021..26998	+	978	WP_001269910.1	hypothetical protein	-
A4V61_RS24975	26995..27420	-	426	WP_000202944.1	hypothetical protein	-
A4V61_RS24980	27488..27934	-	447	WP_001074385.1	hypothetical protein	-
A4V61_RS24985	27959..28813	-	855	WP_032328461.1	replication initiation protein	-
A4V61_RS24990	29877..30128	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
A4V61_RS24995	30118..30399	+	282	WP_000220563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4V61_RS25000	30545..30868	+	324	WP_001181904.1	hypothetical protein	-
A4V61_RS25005	30913..31158	+	246	WP_000356542.1	hypothetical protein	-
A4V61_RS25010	31148..31363	+	216	WP_001180117.1	hypothetical protein	-
A4V61_RS25015	31456..31785	+	330	WP_024222272.1	hypothetical protein	-
A4V61_RS25020	31828..32007	+	180	WP_000439079.1	hypothetical protein	-
A4V61_RS26395	32077..32223	+	147	WP_198406482.1	hypothetical protein	-
A4V61_RS25025	32240..32512	+	273	WP_000167280.1	hypothetical protein	-
A4V61_RS26400	32763..32837	+	75	Protein_44	DNA distortion polypeptide 3	-
A4V61_RS25030	32832..33377	+	546	WP_001426313.1	hypothetical protein	-
A4V61_RS25035	33380..34546	+	1167	WP_024222271.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45056	45056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11021.83 Da
• Isoelectric Point: 10.5938

>T87364 WP_000220563.1 NZ_CP024261:30118-30399 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHSDRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T87364 NZ_CP024261:30118-30399 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATTCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGTTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT87364 WP_000121743.1 NZ_CP024261:29877-30128 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT87364 NZ_CP024261:29877-30128 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S5I301