Detailed information of TA system TA087361

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3853142..3853976
• Replicon: chromosome
• Accession: NZ_CP024260
• Organism: Escherichia coli strain F5656C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: A4V61_RS20325
• Protein ID: WP_140159973.1
• Coordinates: 3853142..3853510 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: A4V61_RS20330
• Protein ID: WP_001285580.1
• Coordinates: 3853608..3853976 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4V61_RS20300	3848269..3849417	-	1149	WP_000905925.1	capsule polysaccharide transporter	-
A4V61_RS20305	3849489..3850472	-	984	WP_032328464.1	KpsF/GutQ family sugar-phosphate isomerase	-
A4V61_RS20310	3851283..3851453	-	171	Protein_3665	IS110 family transposase	-
A4V61_RS20315	3851796..3852638	-	843	WP_001280493.1	DUF4942 domain-containing protein	-
A4V61_RS20320	3852723..3852920	-	198	WP_023563519.1	DUF957 domain-containing protein	-
A4V61_RS26160	3852996..3853145	-	150	Protein_3668	hypothetical protein	-
A4V61_RS20325	3853142..3853510	-	369	WP_140159973.1	TA system toxin CbtA family protein	Toxin
A4V61_RS20330	3853608..3853976	-	369	WP_001285580.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A4V61_RS20335	3854055..3854276	-	222	WP_000691818.1	DUF987 domain-containing protein	-
A4V61_RS20345	3854413..3854592	-	180	WP_001398324.1	hypothetical protein	-
A4V61_RS20350	3854634..3855331	+	698	Protein_3673	IS1 family transposase	-
A4V61_RS20360	3856140..3856364	+	225	WP_001395295.1	transposase	-
A4V61_RS20365	3856366..3856497	-	132	Protein_3675	integrase core domain-containing protein	-
A4V61_RS20370	3856529..3858100	-	1572	WP_000381401.1	IS66 family transposase	-
A4V61_RS20375	3858120..3858467	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-109)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13616.51 Da
• Isoelectric Point: 9.1652

>T87361 WP_140159973.1 NZ_CP024260:c3853510-3853142 [Escherichia coli]
ITQHTRTGGIARPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVSFLVEKYALVRTDQPGFSAC
TRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 369 bp

>T87361 NZ_CP024260:c3853510-3853142 [Escherichia coli]
ATTACCCAACACACACGTACGGGAGGCATCGCGCGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTCCCGGTTGCT
GGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTGAAGCTGGCA
TTTCACTGTGTGATGCGGTGAGCTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTCAGCGCCTGT
ACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGACAACTACAG
AACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13670.43 Da
• Isoelectric Point: 6.9462

>AT87361 WP_001285580.1 NZ_CP024260:c3853976-3853608 [Escherichia coli]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADSYHLNQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT87361 NZ_CP024260:c3853976-3853608 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGAACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA