Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2551032..2551529 Replicon chromosome
Accession NZ_CP024260
Organism Escherichia coli strain F5656C1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A4V61_RS13585 Protein ID WP_001173343.1
Coordinates 2551032..2551205 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag A4V61_RS13590 Protein ID WP_071597404.1
Coordinates 2551233..2551529 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4V61_RS13575 2547697..2549154 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
A4V61_RS13580 2549391..2550908 + 1518 WP_001295074.1 lysine--tRNA ligase -
A4V61_RS13585 2551032..2551205 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A4V61_RS13590 2551233..2551529 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A4V61_RS13600 2551756..2552028 - 273 WP_000405647.1 N-acetyltransferase -
A4V61_RS13605 2552040..2552270 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
A4V61_RS13610 2552451..2554082 + 1632 WP_001216490.1 sensor histidine kinase -
A4V61_RS13615 2554079..2554798 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - vipB/tssC / vipA/tssB 2473587..2596659 123072


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87356 WP_001173343.1 NZ_CP024260:c2551205-2551032 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87356 NZ_CP024260:c2551205-2551032 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT87356 WP_071597404.1 NZ_CP024260:c2551529-2551233 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87356 NZ_CP024260:c2551529-2551233 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References