Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1715718..1716336 Replicon chromosome
Accession NZ_CP024260
Organism Escherichia coli strain F5656C1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A4V61_RS09135 Protein ID WP_001291435.1
Coordinates 1716118..1716336 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID -
Locus tag A4V61_RS09130 Protein ID WP_000344789.1
Coordinates 1715718..1716092 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4V61_RS09120 1710807..1712000 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
A4V61_RS09125 1712023..1715172 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
A4V61_RS09130 1715718..1716092 + 375 WP_000344789.1 Hha toxicity modulator TomB Antitoxin
A4V61_RS09135 1716118..1716336 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A4V61_RS09140 1716508..1717059 + 552 WP_000102564.1 maltose O-acetyltransferase -
A4V61_RS09145 1717175..1717645 + 471 WP_000136192.1 YlaC family protein -
A4V61_RS09150 1717809..1719359 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A4V61_RS09155 1719401..1719754 - 354 WP_000878143.1 DUF1428 domain-containing protein -
A4V61_RS09165 1720133..1720444 + 312 WP_000409911.1 MGMT family protein -
A4V61_RS09170 1720475..1721047 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87353 WP_001291435.1 NZ_CP024260:1716118-1716336 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87353 NZ_CP024260:1716118-1716336 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14569.44 Da        Isoelectric Point: 4.7395

>AT87353 WP_000344789.1 NZ_CP024260:1715718-1716092 [Escherichia coli]
MDEYSPKRHDIAQLKFLCEILYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87353 NZ_CP024260:1715718-1716092 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAATCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References