Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 725066..725704 Replicon chromosome
Accession NZ_CP024260
Organism Escherichia coli strain F5656C1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A4V61_RS03755 Protein ID WP_000813794.1
Coordinates 725528..725704 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A4V61_RS03750 Protein ID WP_001270286.1
Coordinates 725066..725482 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4V61_RS03730 720218..721159 - 942 WP_001251313.1 ABC transporter permease -
A4V61_RS03735 721160..722173 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
A4V61_RS03740 722191..723336 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
A4V61_RS03745 723581..724987 - 1407 WP_000760586.1 PLP-dependent aminotransferase family protein -
A4V61_RS03750 725066..725482 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A4V61_RS03755 725528..725704 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A4V61_RS03760 725926..726150 + 225 WP_071996615.1 YncJ family protein -
A4V61_RS03765 726248..728209 - 1962 WP_001355676.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A4V61_RS03770 728282..728818 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
A4V61_RS03775 728871..730085 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T87352 WP_000813794.1 NZ_CP024260:c725704-725528 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T87352 NZ_CP024260:c725704-725528 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT87352 WP_001270286.1 NZ_CP024260:c725482-725066 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT87352 NZ_CP024260:c725482-725066 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References