Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4417066..4417642 Replicon chromosome
Accession NZ_CP024257
Organism Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag COG37_RS23085 Protein ID WP_001297643.1
Coordinates 4417355..4417642 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag COG37_RS23080 Protein ID WP_000063156.1
Coordinates 4417066..4417368 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG37_RS23065 4413662..4415812 + 2151 WP_001736090.1 pyruvate/proton symporter BtsT -
COG37_RS23070 4415862..4416065 + 204 WP_000467859.1 YbdD/YjiX family protein -
COG37_RS23075 4416076..4417032 + 957 WP_001297640.1 GTPase -
COG37_RS23080 4417066..4417368 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
COG37_RS23085 4417355..4417642 - 288 WP_001297643.1 BrnT family toxin Toxin
COG37_RS23090 4417841..4418538 - 698 Protein_4204 IS1 family transposase -
COG37_RS23095 4418595..4418690 + 96 Protein_4205 dienelactone hydrolase family protein -
COG37_RS23100 4418836..4420068 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
COG37_RS23105 4420109..4421389 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T87330 WP_001297643.1 NZ_CP024257:c4417642-4417355 [Escherichia coli O25:H16]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T87330 NZ_CP024257:c4417642-4417355 [Escherichia coli O25:H16]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT87330 WP_000063156.1 NZ_CP024257:c4417368-4417066 [Escherichia coli O25:H16]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT87330 NZ_CP024257:c4417368-4417066 [Escherichia coli O25:H16]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References