Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2755220..2755858 Replicon chromosome
Accession NZ_CP024257
Organism Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag COG37_RS14190 Protein ID WP_000813794.1
Coordinates 2755682..2755858 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag COG37_RS14185 Protein ID WP_001270286.1
Coordinates 2755220..2755636 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG37_RS14165 2750372..2751313 - 942 WP_001251313.1 ABC transporter permease -
COG37_RS14170 2751314..2752327 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
COG37_RS14175 2752345..2753490 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
COG37_RS14180 2753735..2755141 - 1407 WP_001735217.1 PLP-dependent aminotransferase family protein -
COG37_RS14185 2755220..2755636 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
COG37_RS14190 2755682..2755858 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
COG37_RS14195 2756080..2756310 + 231 WP_000494244.1 YncJ family protein -
COG37_RS14200 2756402..2758363 - 1962 WP_001735216.1 23S rRNA 5-hydroxycytidine C2501 synthase -
COG37_RS14205 2758436..2758972 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
COG37_RS14210 2759025..2760239 + 1215 WP_071598249.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T87326 WP_000813794.1 NZ_CP024257:c2755858-2755682 [Escherichia coli O25:H16]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T87326 NZ_CP024257:c2755858-2755682 [Escherichia coli O25:H16]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT87326 WP_001270286.1 NZ_CP024257:c2755636-2755220 [Escherichia coli O25:H16]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT87326 NZ_CP024257:c2755636-2755220 [Escherichia coli O25:H16]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References