Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3234787..3235425 Replicon chromosome
Accession NZ_CP024256
Organism Escherichia coli strain ATCC 43886

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CS032_RS18480 Protein ID WP_000813794.1
Coordinates 3234787..3234963 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CS032_RS18485 Protein ID WP_001270286.1
Coordinates 3235009..3235425 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS032_RS18460 3230406..3231620 - 1215 WP_071598249.1 BenE family transporter YdcO -
CS032_RS18465 3231673..3232209 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
CS032_RS18470 3232282..3234243 + 1962 WP_001735216.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CS032_RS18475 3234335..3234565 - 231 WP_000494244.1 YncJ family protein -
CS032_RS18480 3234787..3234963 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CS032_RS18485 3235009..3235425 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CS032_RS18490 3235504..3236910 + 1407 WP_001735217.1 PLP-dependent aminotransferase family protein -
CS032_RS18495 3237155..3238300 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
CS032_RS18500 3238318..3239331 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
CS032_RS18505 3239332..3240273 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T87298 WP_000813794.1 NZ_CP024256:3234787-3234963 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T87298 NZ_CP024256:3234787-3234963 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT87298 WP_001270286.1 NZ_CP024256:3235009-3235425 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT87298 NZ_CP024256:3235009-3235425 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References