Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2133378..2133996 Replicon chromosome
Accession NZ_CP024256
Organism Escherichia coli strain ATCC 43886

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CS032_RS12500 Protein ID WP_001291435.1
Coordinates 2133378..2133596 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CS032_RS12505 Protein ID WP_000344800.1
Coordinates 2133622..2133996 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS032_RS12465 2128667..2129239 + 573 WP_000779831.1 YbaY family lipoprotein -
CS032_RS12470 2129270..2129581 - 312 WP_000409911.1 MGMT family protein -
CS032_RS12480 2129960..2130313 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CS032_RS12485 2130355..2131905 - 1551 WP_001727568.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CS032_RS12490 2132069..2132539 - 471 WP_000136192.1 YlaC family protein -
CS032_RS12495 2132655..2133206 - 552 WP_001727569.1 maltose O-acetyltransferase -
CS032_RS12500 2133378..2133596 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CS032_RS12505 2133622..2133996 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CS032_RS12510 2134542..2137691 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CS032_RS12515 2137714..2138907 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87297 WP_001291435.1 NZ_CP024256:c2133596-2133378 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87297 NZ_CP024256:c2133596-2133378 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87297 WP_000344800.1 NZ_CP024256:c2133996-2133622 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87297 NZ_CP024256:c2133996-2133622 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References