Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 25029..25588 Replicon plasmid unnamed1
Accession NZ_CP024254
Organism Escherichia coli strain ATCC 43886

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag CS032_RS00245 Protein ID WP_000421257.1
Coordinates 25029..25304 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag CS032_RS00250 Protein ID WP_001178089.1
Coordinates 25304..25588 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS032_RS00210 20261..20947 - 687 WP_001284954.1 winged helix-turn-helix domain-containing protein -
CS032_RS00215 20955..21341 - 387 WP_000460651.1 hypothetical protein -
CS032_RS00220 21334..21654 - 321 WP_000562370.1 antibiotic biosynthesis monooxygenase -
CS032_RS00225 22098..23303 + 1206 WP_000599533.1 sodium/glutamate symporter -
CS032_RS00230 23534..23653 + 120 Protein_35 LysR family transcriptional regulator -
CS032_RS00235 23669..24877 - 1209 WP_001352368.1 IS4-like element ISVsa5 family transposase -
CS032_RS00245 25029..25304 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CS032_RS00250 25304..25588 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
CS032_RS00265 26493..27365 - 873 WP_000729438.1 incFII family plasmid replication initiator RepA -
CS032_RS00280 28069..28710 + 642 WP_000335849.1 transcription termination factor NusG -
CS032_RS00285 28851..29513 + 663 WP_000154142.1 hypothetical protein -
CS032_RS00295 29780..30400 + 621 WP_000176497.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / sul2 - 1..107732 107732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T87284 WP_000421257.1 NZ_CP024254:c25304-25029 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T87284 NZ_CP024254:c25304-25029 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT87284 WP_001178089.1 NZ_CP024254:c25588-25304 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT87284 NZ_CP024254:c25588-25304 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References