Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 97249..97823 Replicon plasmid unnamed3
Accession NZ_CP024248
Organism Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)


Gene name relE Uniprot ID A0A0S3PMZ8
Locus tag CQS47_RS25675 Protein ID WP_001595119.1
Coordinates 97249..97575 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0S3PN98
Locus tag CQS47_RS25680 Protein ID WP_001595120.1
Coordinates 97572..97823 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQS47_RS25650 93868..94845 - 978 WP_001595112.1 RepB family plasmid replication initiator protein -
CQS47_RS25665 95718..96849 - 1132 Protein_121 IS91 family transposase -
CQS47_RS25670 96849..97145 - 297 WP_000766063.1 hypothetical protein -
CQS47_RS25675 97249..97575 - 327 WP_001595119.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CQS47_RS25680 97572..97823 - 252 WP_001595120.1 hypothetical protein Antitoxin
CQS47_RS25690 98377..99243 + 867 WP_001595122.1 AAA family ATPase -
CQS47_RS25695 99243..100274 + 1032 WP_001595123.1 ParB/RepB/Spo0J family partition protein -
CQS47_RS25700 100298..100711 + 414 WP_032159708.1 hypothetical protein -
CQS47_RS26470 100708..101031 + 324 WP_000143877.1 hypothetical protein -
CQS47_RS25710 101070..102344 - 1275 WP_001595125.1 Y-family DNA polymerase -
CQS47_RS25715 102344..102766 - 423 WP_001595126.1 translesion error-prone DNA polymerase V autoproteolytic subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - estIa / faeE / faeD / cofA / cssA 1..138289 138289


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12187.44 Da        Isoelectric Point: 10.4373

>T87283 WP_001595119.1 NZ_CP024248:c97575-97249 [Escherichia coli O27:H7]
MTYTVKFRDDALKEWLKLDKAIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAIGK
RERSDVYNLASRVGSLAAAACHGLKLPL

Download         Length: 327 bp

>T87283 NZ_CP024248:c97575-97249 [Escherichia coli O27:H7]
ATGACTTACACAGTCAAATTCAGGGATGATGCACTGAAAGAATGGCTGAAACTGGATAAGGCCATTCAACAGCAATTTGC
TAAAAAGCTAAAGAAGTGCAGTGAAAATCCTCATATTCCCTCTGCTAAGCTGCGCGGGATAAAAGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATAGATGATCAATTAATTATCGCTGTTGTTGCTATTGGGAAA
CGTGAACGTAGTGATGTTTATAATCTTGCCAGCCGAGTAGGCAGCCTGGCGGCTGCGGCTTGTCATGGACTGAAATTACC
GTTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9144.43 Da        Isoelectric Point: 4.2975

>AT87283 WP_001595120.1 NZ_CP024248:c97823-97572 [Escherichia coli O27:H7]
MPNIILTDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYGKMLDAMDDQELVKLVAERSNQKLHDVDLD
SYL

Download         Length: 252 bp

>AT87283 NZ_CP024248:c97823-97572 [Escherichia coli O27:H7]
ATGCCTAACATCATTCTTACCGACACAAGCGCCAGTGTAAGCGAACTTAAGAAAAACCCGATGGCTACAGTCAGCGCTGG
TGATGGCTACCCAGTTGCTATTCTGAACCGCAACCAGCCAGCCTTTTACTGCGTACCCGCAGAGTTGTACGGAAAGATGC
TCGATGCCATGGACGATCAGGAACTGGTTAAGCTAGTCGCCGAACGCAGCAACCAGAAGCTGCACGATGTGGATCTGGAT
AGCTACTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S3PMZ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3PN98

References