Detailed information of TA system TA087276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 26871..27514
• Replicon: plasmid unnamed1
• Accession: NZ_CP024246
• Organism: Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CQS47_RS24485
• Protein ID: WP_099590313.1
• Coordinates: 26871..27287 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: E9XUD3
• Locus tag: CQS47_RS24490
• Protein ID: WP_001261279.1
• Coordinates: 27284..27514 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQS47_RS24455	22734..23933	+	1200	Protein_25	IS3 family transposase	-
CQS47_RS26440	24137..24703	+	567	WP_000587873.1	hypothetical protein	-
CQS47_RS24465	24771..24980	-	210	Protein_27	transposase domain-containing protein	-
CQS47_RS24470	25026..25169	+	144	Protein_28	transposase	-
CQS47_RS24475	25540..26148	-	609	WP_000515889.1	hypothetical protein	-
CQS47_RS24485	26871..27287	-	417	WP_099590313.1	type II toxin-antitoxin system VapC family toxin	Toxin
CQS47_RS24490	27284..27514	-	231	WP_001261279.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CQS47_RS24495	28118..28531	+	414	WP_000465051.1	hypothetical protein	-
CQS47_RS24500	28533..29318	+	786	WP_001164197.1	site-specific integrase	-
CQS47_RS24505	29902..30534	+	633	WP_000312331.1	ParA family protein	-
CQS47_RS26445	30534..30908	+	375	WP_000752652.1	hypothetical protein	-
CQS47_RS24515	31147..32121	+	975	WP_001217836.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36948	36948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15178.61 Da
• Isoelectric Point: 7.2929

>T87276 WP_099590313.1 NZ_CP024246:c27287-26871 [Escherichia coli O27:H7]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHHIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T87276 NZ_CP024246:c27287-26871 [Escherichia coli O27:H7]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCATATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8761.90 Da
• Isoelectric Point: 4.6310

>AT87276 WP_001261279.1 NZ_CP024246:c27514-27284 [Escherichia coli O27:H7]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPAWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT87276 NZ_CP024246:c27514-27284 [Escherichia coli O27:H7]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAAGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGCCCGGCCTGGGGCTCGTTCGCGCAGCTCG
AGAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3U0EQM4