Detailed information of TA system TA087274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4317915..4318752
• Replicon: chromosome
• Accession: NZ_CP024245
• Organism: Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: CQS47_RS22355
• Protein ID: WP_000227784.1
• Coordinates: 4318210..4318752 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CQS47_RS22350
• Protein ID: WP_001297137.1
• Coordinates: 4317915..4318226 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQS47_RS22325	4312935..4313882	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
CQS47_RS22330	4313904..4315895	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CQS47_RS22335	4315885..4316499	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CQS47_RS22340	4316499..4316828	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CQS47_RS22345	4316840..4317730	+	891	WP_000971336.1	heme o synthase	-
CQS47_RS22350	4317915..4318226	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CQS47_RS22355	4318210..4318752	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
CQS47_RS22360	4318808..4319743	-	936	WP_001297127.1	sel1 repeat family protein	-
CQS47_RS22365	4320151..4321515	+	1365	WP_001000978.1	MFS transporter	-
CQS47_RS22370	4321643..4322134	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CQS47_RS22375	4322302..4323213	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T87274 WP_000227784.1 NZ_CP024245:4318210-4318752 [Escherichia coli O27:H7]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T87274 NZ_CP024245:4318210-4318752 [Escherichia coli O27:H7]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT87274 WP_001297137.1 NZ_CP024245:4317915-4318226 [Escherichia coli O27:H7]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT87274 NZ_CP024245:4317915-4318226 [Escherichia coli O27:H7]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3