Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4283839..4284457 Replicon chromosome
Accession NZ_CP024245
Organism Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CQS47_RS22180 Protein ID WP_001291435.1
Coordinates 4284239..4284457 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CQS47_RS22175 Protein ID WP_000344800.1
Coordinates 4283839..4284213 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQS47_RS22165 4278928..4280121 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CQS47_RS22170 4280144..4283293 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CQS47_RS22175 4283839..4284213 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CQS47_RS22180 4284239..4284457 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CQS47_RS22185 4284628..4285179 + 552 WP_000102578.1 maltose O-acetyltransferase -
CQS47_RS22190 4285295..4285765 + 471 WP_000136192.1 YlaC family protein -
CQS47_RS22195 4285929..4287479 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CQS47_RS22200 4287521..4287874 - 354 WP_000878141.1 DUF1428 family protein -
CQS47_RS22210 4288253..4288564 + 312 WP_000409911.1 MGMT family protein -
CQS47_RS22215 4288595..4289167 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87273 WP_001291435.1 NZ_CP024245:4284239-4284457 [Escherichia coli O27:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87273 NZ_CP024245:4284239-4284457 [Escherichia coli O27:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87273 WP_000344800.1 NZ_CP024245:4283839-4284213 [Escherichia coli O27:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87273 NZ_CP024245:4283839-4284213 [Escherichia coli O27:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References