Detailed information of TA system TA087261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2151819..2152444
• Replicon: chromosome
• Accession: NZ_CP024245
• Organism: Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CQS47_RS11045
• Protein ID: WP_000911330.1
• Coordinates: 2152046..2152444 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CQS47_RS11040
• Protein ID: WP_000450524.1
• Coordinates: 2151819..2152046 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQS47_RS11015	2147622..2148092	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CQS47_RS11020	2148092..2148664	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CQS47_RS11025	2148810..2149688	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CQS47_RS11030	2149705..2150739	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
CQS47_RS11035	2150952..2151665	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CQS47_RS11040	2151819..2152046	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CQS47_RS11045	2152046..2152444	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CQS47_RS11050	2152591..2153454	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CQS47_RS11055	2153469..2155484	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
CQS47_RS11060	2155558..2156256	+	699	WP_000679823.1	esterase	-
CQS47_RS11065	2156366..2156566	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T87261 WP_000911330.1 NZ_CP024245:2152046-2152444 [Escherichia coli O27:H7]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T87261 NZ_CP024245:2152046-2152444 [Escherichia coli O27:H7]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT87261 WP_000450524.1 NZ_CP024245:2151819-2152046 [Escherichia coli O27:H7]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT87261 NZ_CP024245:2151819-2152046 [Escherichia coli O27:H7]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33