Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1690207..1690861 Replicon chromosome
Accession NZ_CP024245
Organism Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag CQS47_RS08770 Protein ID WP_000244772.1
Coordinates 1690454..1690861 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CQS47_RS08765 Protein ID WP_000354046.1
Coordinates 1690207..1690473 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQS47_RS08740 1685376..1686119 + 744 WP_000951964.1 SDR family oxidoreductase -
CQS47_RS08745 1686176..1687609 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
CQS47_RS08750 1687654..1687965 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CQS47_RS08755 1688129..1688788 + 660 WP_000250274.1 hemolysin III family protein -
CQS47_RS08760 1688984..1689964 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CQS47_RS08765 1690207..1690473 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CQS47_RS08770 1690454..1690861 + 408 WP_000244772.1 protein YgfX Toxin
CQS47_RS08775 1690901..1691422 - 522 WP_001055874.1 flavodoxin FldB -
CQS47_RS08780 1691534..1692430 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CQS47_RS08785 1692455..1693165 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CQS47_RS08790 1693171..1694904 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T87259 WP_000244772.1 NZ_CP024245:1690454-1690861 [Escherichia coli O27:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87259 NZ_CP024245:1690454-1690861 [Escherichia coli O27:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87259 WP_000354046.1 NZ_CP024245:1690207-1690473 [Escherichia coli O27:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87259 NZ_CP024245:1690207-1690473 [Escherichia coli O27:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References